Local Axion Cosmic Strings from Superstrings

Axionic cosmic string solutions are investigated in a superstring motivated model with a pseudo-anomalous U(1) gauge symmetry. The inclusion of a gauge field and spatially varying dilaton allow local defect solutions with finite energy per unit length to be found. Fermion zero modes (whose presence is implied by supersymmetry) are also analysed. The corresponding fermion currents suggest strong cosmological bounds on the model. It is shown that the unusual form of the axion strings weakens these bounds. Other cosmological constraints on the underlying theory are also discussed.Comment: 17 page

A tight squeeze: How do we make sense of small changes in microvascular diameter?

The brain is an energetically demanding tissue which, to function adequately, requires constant fine tuning of its supporting blood flow, and hence energy supply. Whilst blood flow was traditionally believed to be regulated only by vascular smooth muscle cells on arteries and arterioles supplying the brain, recent work has suggested a critical role for capillary pericytes, which are also contractile. This concept has evoked some controversy, especially over the relative contributions of arterioles and capillaries to the control of cerebral blood flow. Here we outline why pericytes are in a privileged position to control cerebral blood flow. First we discuss the evidence, and fundamental equations, which describe how the small starting diameter of capillaries, compared to upstream arterioles, confers a potentially greater control by capillary pericytes than by arterioles over total cerebral vascular resistance. Then we suggest that the faster time frame over which low branch order capillary pericytes dilate in response to local energy demands provides a niche role for pericytes to regulate blood flow compared to slower responding arterioles. Finally, we discuss the role of pericytes in capillary stalling, whereby pericyte contraction appears to facilitate a transient stall of circulating blood cells, exacerbating the effect of pericytes upon cerebral blood flow

A human embryonic kidney 293T cell line mutated at the Golgi -mannosidase II locus

Disruption of Golgi -mannosidase II activity can result in type II congenital dyserythropoietic anemia and can induce lupus-like autoimmunity in mice. Here, we isolate a mutant human embryonic kidney (HEK) 293T cell line, called Lec36, that displays sensitivity to ricin that lies between the parental HEK 293T cells, whose secreted and membrane-expressed proteins are dominated by complex-type glycosylation, and 293S Lec1 cells, which only produce oligomannose-type N-linked glycans. The stem cell marker, 19A, was transiently expressed in the HEK 293T Lec36 cells, and in parental HEK 293T cells with and without the potent Golgi -mannosidase II inhibitor, swainsonine. Negative-ion nano-electrospray ionization mass spectra of the 19A N-linked glycans from HEK 293T Lec36 and swainsonine-treated HEK 293T cells were qualitatively indistinguishable and, as shown by collision-induced dissociation spectra, dominated by hybrid-type glycosylation. Nucleotide sequencing revealed mutations in each allele of MAN2A1, the gene encoding Golgi -mannosidase II: a point mutation in one allele mapping to the active site and an in-frame deletion of twelve-nucleotides in the other. Expression of wild-type but not the mutant MAN2A1 alleles in Lec36 cells restored processing of the 19A reporter glycoprotein to complex-type glycosylation. The Lec36 cell line will be useful for expressing therapeutic glycoproteins with hybrid-type glycans and provides a sensitive host for detecting mutations in human MAN2A1 causing type II congenital dyserythropoietic anemia

L^2-Betti numbers of hypersurface complements

In \cite{DJL07} it was shown that if \scra is an affine hyperplane arrangement in \C^n, then at most one of the $L^2$--Betti numbers b_i^{(2)}(\C^n\sm \scra,\id) is non--zero. In this note we prove an analogous statement for complements of complex affine hyperurfaces in general position at infinity. Furthermore, we recast and extend to this higher-dimensional setting results of \cite{FLM,LM06} about $L^2$--Betti numbers of plane curve complements.Comment: 10 pages; comments are very welcome; v2: minor clarifications added in the tex

Use of the α-mannosidase I inhibitor kifunensine allows the crystallization of apo CTLA-4 homodimer produced in long-term cultures of Chinese hamster ovary cells

The α-mannosidase I inhibitor kifunensine inhibited N-glycan processing in long-term cultures of Chinese hamster ovary cells, allowing deglycosylation and crystallization of the homodimeric extracellular region of the inhibitory glycoprotein receptor CTLA-4 (CD152)

RNA Sequencing Reveals Novel Transcripts from Sympathetic Stellate Ganglia During Cardiac Sympathetic Hyperactivity.

Cardiovascular disease is the most prevalent age-related illness worldwide, causing approximately 15 million deaths every year. Hypertension is central in determining cardiovascular risk and is a strong predictive indicator of morbidity and mortality; however, there remains an unmet clinical need for disease-modifying and prophylactic interventions. Enhanced sympathetic activity is a well-established contributor to the pathophysiology of hypertension, however the cellular and molecular changes that increase sympathetic neurotransmission are not known. The aim of this study was to identify key changes in the transcriptome in normotensive and spontaneously hypertensive rats. We validated 15 of our top-scoring genes using qRT-PCR, and network and enrichment analyses suggest that glutamatergic signalling plays a key role in modulating Ca2+ balance within these ganglia. Additionally, phosphodiesterase activity was found to be altered in stellates obtained from the hypertensive rat, suggesting that impaired cyclic nucleotide signalling may contribute to disturbed Ca2+ homeostasis and sympathetic hyperactivity in hypertension. We have also confirmed the presence of these transcripts in human donor stellate samples, suggesting that key genes coupled to neurotransmission are conserved. The data described here may provide novel targets for future interventions aimed at treating sympathetic hyperactivity associated with cardiovascular disease and other dysautonomias

The Spectral Types of White Dwarfs in Messier 4

We present the spectra of 24 white dwarfs in the direction of the globular cluster Messier 4 obtained with the Keck/LRIS and Gemini/GMOS spectrographs. Determining the spectral types of the stars in this sample, we find 24 type DA and 0 type DB (i.e., atmospheres dominated by hydrogen and helium respectively). Assuming the ratio of DA/DB observed in the field with effective temperature between 15,000 - 25,000 K, i.e., 4.2:1, holds for the cluster environment, the chance of finding no DBs in our sample due simply to statistical fluctuations is only 6 X 10^(-3). The spectral types of the ~100 white dwarfs previously identified in open clusters indicate that DB formation is strongly suppressed in that environment. Furthermore, all the ~10 white dwarfs previously identified in other globular clusters are exclusively type DA. In the context of these two facts, this finding suggests that DB formation is suppressed in the cluster environment in general. Though no satisfactory explanation for this phenomenon exists, we discuss several possibilities.Comment: Accepted for Publication in Astrophys. J. 11 pages including 4 figures and 2 tables (journal format

Neutrinos: Heralds of New Physics

The central role of neutrinos in the determination of fundamental interactions is reviewed. The recent SuperKamiokande discovery of neutrino mass gives an {\it aper\c cu} of physics at short distances, and tests theories of flavor. Quark-lepton symmetries, derived from grand unification and/or string theories, can help determine the standard model parameters in the neutrino sector.Comment: Invited Talk at TAUP99, Paris, September 1999. Latex, 10 page